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Image Search Results
Journal: Applied and Environmental Microbiology
Article Title: A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family
doi: 10.1128/AEM.01383-17
Figure Lengend Snippet: SDS-PAGE of purification steps and deglycosylation of Colletotrichum graminicola RaOx. (A) Figure lanes show molecular mass marker proteins in kDa. Lane 1, concentrated culture supernatant (21 μg protein); lane 2, pooled protein fractions after phenyl Sepharose (3 μg); lane 3, pooled protein after DEAE (3 μg). Total protein in each lane in indicated in parentheses. (B) The CgRaOx enzyme was deglycosylated with Endo F1 and analyzed before (lane 1) and after the treatment (lane 2). The molecular mass marker proteins (250, 150, 100, 75, 50, 37, and 25 kDa) are in lane Mw. The proteins were visualized using the Bio-Rad's Criterion stain-free gel imaging system (Bio-Rad). The gels shown have been spliced for labeling purposes.
Article Snippet: The purity of the enzymes was monitored by SDS-PAGE (10% Criterion stain-free gel; Bio-Rad), and the proteins were visualized using the
Techniques: SDS Page, Purification, Marker, Staining, Imaging, Labeling